Phylogeography and Molecular Evolution of Potato virus Y

نویسندگان

  • José M. Cuevas
  • Agnès Delaunay
  • Johan C. Visser
  • Dirk U. Bellstedt
  • Emmanuel Jacquot
  • Santiago F. Elena
چکیده

Potato virus Y (PVY) is an important plant pathogen, whose host range includes economically important crops such as potato, tobacco, tomato, and pepper. PVY presents three main strains (PVY(O), PVY(N) and PVY(C)) and several recombinant forms. PVY has a worldwide distribution, yet the mechanisms that promote and maintain its population structure and genetic diversity are still unclear. In this study, we used a pool of 77 complete PVY genomes from isolates collected worldwide. After removing the effect of recombination in our data set, we used bayesian techniques to study the influence of geography and host species in both PVY population structure and dynamics. We have also performed selection and covariation analyses to identify evolutionarily relevant amino acid residues. Our results show that both geographic and host-driven adaptations explain PVY diversification. Furthermore, purifying selection is the main force driving PVY evolution, although some indications of positive selection accounted for the diversification of the different strains. Interestingly, the analysis of P3N-PIPO, a recently described gene in potyviruses, seems to show a variable length among the isolates analyzed, and this variability is explained, in part, by host-driven adaptation.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Molecular evolution and phylogeography of potato virus Y based on the CP gene.

Potato virus Y (PVY) is an important plant pathogen with a wide host range that includes, among others, potato, tobacco, tomato and pepper. The coat protein (CP) of PVY has been commonly used in phylogenetic studies for strain classification. In this study, we used a pool of 292 CP sequences from isolates collected worldwide. After detecting and removing recombinant sequences, we applied Bayesi...

متن کامل

Induction of Resistance to Potato Virus Y (PVY) Using Hairpin Construct of Coat Protein

Potato virus Y (PVY) is one of the most damaging viruses of potato plants which infecting most cultivars and causing significant yield and economical losses. The application of the concept of pathogen derived resistance opened new horizons for the development of virus-resistant plants. This research was carried out to study RNA silencing to engineered potato plants that are resistant to potato ...

متن کامل

Heterologous Expression of Potato Virus Y Coat Protein, Isolate Pot187

Background: The advent of recombinant DNA technology has facilitated heterologous expression of proteins from various sources in different host systems including Escherichia coli. If a plant virus coat protein is expressed in the bacterium it can be used as the antigen for antibody preparation. Such a recombinant antigen preparation can be particularly useful where equipment such as ultracentri...

متن کامل

Investigation on the reaction of Iranian oriental and semi oriental tobacco genotypes to Potato Virus Y (PVY) under controlled conditions

Potato virus Y (PVY) as one of the serious diseases on tobacco (Nicotiana tabacum L.), reduces leaf yield and its quality. Use of re-sistant genotypes is being considered as the most effective, economical and a safe way to reduce damages of the virus. In the present study, the response of 90 oriental and semi-oriental tobacco genotypes was evaluated against PVY using randomized complete block d...

متن کامل

Detecting past recombination events in Potato virus Y genomic sequences using statistical methods

Molecular evolution of RNA viruses: Plant RNA viruses evolve more rapidly than the DNA of their plant hosts due to a higher mutation rate. Mutational processes include nucleotide substitution that replaces one nucleotide by another, and insertions and/or deletions that result in sequence length changes. In addition to mutation, inter-strain recombination can produce new mosaic strains9. It is i...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 7  شماره 

صفحات  -

تاریخ انتشار 2012